Author Topic: Tip of the day: Surface preparation for small molecules  (Read 802 times)

GE Marie W

  • Administrator
  • Newbie
  • *****
  • Posts: 27
Tip of the day: Surface preparation for small molecules
« on: January 25, 2016, 07:35:46 AM »
Tipof the day: Surface preparation for small molecules (low molecular weight compounds)

  • Sensor chip CM5 is recommended. If sufficient ligand levels cannot be obtained, then use the high capacity Sensor Chip CM7. It provides a high surface binding capacity and relatively low levels of non-specific binding.
  • Generally, direct immobilization of target proteins is recommended.
  • Capture might be an alternative for small molecule screens (not recommended for fragment screening though): Capture minimizes time spent on finding suitable immobilization and regeneration conditions. Capture will require larger volumes of target molecule. Due to variations in capture levels, low responses can be difficult to compare reliably.
  • Immobilization levels should be high (typically ~10 000 RU for medium-sized protein targets).