Author Topic: Capture of goat and rabbit antibodies  (Read 885 times)

Rick

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Capture of goat and rabbit antibodies
« on: November 30, 2015, 04:18:33 AM »
Hi,

I have been using your kit for capturing mouse antibodies but now have some goat and rabbit antibodies. Any suggestions how to get a capture assay for those?

Best regards,
Rick

GE Asa

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Re: Capture of goat and rabbit antibodies
« Reply #1 on: December 02, 2015, 01:09:58 AM »
Hi,

the Protein A chip can be used for the rabbit antibodies (note that with protein A in rare cases, for any species, certain antibodies bind less well).

For the goat antibodies Protein G should work well, and give stable capture. Protein G can be amine coupled using pH 4 and regenerated with 30 s of 10 mM glycine, pH 1.5.

Best wishes,
Asa