General Category > 2-D Electrophoresis

2D electrophoresis western blot? (Yes, two techniques in one....)

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Sole:
Hello everyone, I want to know if you have performed a 2D gel and transfered the proteins in the gel to a PVDF membrane to incubate then with anti-Phosphoprotein ab.
I need to do something like this with cerebellum tissue and I am looking for a protocol
Thank you very much!

Soledad.-

Sjouke Hoving:
Hello,
You can just run a standard 2D gel as described here in the forum. After running the gel, you can continue with any standard blotting procedure.
In our hands a wet transfer gives best results. Which format are you planning to run?

sjouke

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