Author Topic: how do carrier ampholytes establish pH gradient in IEF  (Read 1865 times)

researcher123

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how do carrier ampholytes establish pH gradient in IEF
« on: August 27, 2013, 09:53:25 PM »
Hi all!

I understand that carrier ampholytes are a range of compounds that create pH gradient in IEF gels- they have different pIs and have a high buffering capacity at their respective pIs. My question is- how do these compounds have a buffering capacity at their pI?? How do they actually create the pH gradient and make it stable???

Thanks!!!   :)