Author Topic: Loading Controls  (Read 4068 times)

fract

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Loading Controls
« on: March 05, 2012, 09:23:28 AM »
Does anyone have any information on a protein that can be used as a loading control for serum western blots
thanks

GE Maria

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Re: Loading Controls
« Reply #1 on: March 07, 2012, 05:23:28 AM »
Hi,

If you are going to run a Western blott on a serum sample you could use transferrin as a loading control. Albumin could also work if you dilute your primary and secondary antibodies extensively otherwise you risk to get a “big blob” since serum is very rich in albumin.

Good luck!
GE Maria