Author Topic: Speckled background  (Read 2632 times)

GE Maria

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Speckled background
« on: February 07, 2012, 03:02:56 AM »
If I have problems with speckled background what could be the cause?

Aggregates in the blocking agent could cause this so ensure that the blocking agent is completely dissolved in the buffer and that the membrane is fully immersed in the blocking solution throughout the incubation.
This could also be due to aggregates in HRP-conjugated secondary antibody, always use a fresh solution of high quality.
Exposure equipment not clean or adequate

Hope this could be to some help!
Best regards,
GE Maria