Author Topic: 2D Quant Kit  (Read 1140 times)

reetta

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2D Quant Kit
« on: January 27, 2010, 05:00:00 PM »
Hey!

I was doing protein assay with 2D quant kit, but my samples contained 15N isotope and i got quite high or just very good protein concentration. But i dont believe that my cell disruption was so successful. Does anyone has a experience with this protein assay and isotope labeled samples? Does 2D quant kit suits with 15N isotope?

Take care!
reetta


Sjouke Hoving

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Re: 2D Quant Kit (reetta)
« Reply #1 on: January 28, 2010, 05:00:00 PM »
I doubt that an 15N isotope has any influence on a protein quantitation kit. But I presume you are using cells, so why don't you use cell counting. For B-cells for example 10E7 cells is corresponding to 1 mg protein. The quant kits in the lysis buffer for 2D gels are quite tricky, at least in our hands.

sjouke