Author Topic: What's the difference between 29:1 acrylamide:bis and 37.5:1 acrylamide:bis?  (Read 12848 times)

libby

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Hi everyone,

As the topic title, I wonder if there is any difference between these two types of acrylamide in 2nd dimension of 2D. I noticed that the GE 2DE manual book recommended to use 30% 37.5:1 acrylamide:bis stock. However, another senior member in our lab insisted to use 40% 29:1 acrylamide:bis stock. To be honest, I have used both and found that there were some differences in protein mobility between these two recipes. What have you been using?  I appreciate your suggestions.

Libby


cchan

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Re: What's the difference between 29:1 acrylamide:bis and 37.5:1 ... (libby)
« Reply #1 on: January 15, 2007, 05:00:00 PM »
Hi Libby,

The ratios refer to the amount of acrylamide to bis-acrylamide and will affect the crosslinker in the gel. We typically use the 37.5:1 ratio. By using different ratios, you can change the pore size of the polyacrylamide gel which will change the mobility of the proteins running through it. A more detailed explanation is available from the National Diagnostics website:

http://nationaldiagnostics.com/article_info.php/articles_id/6" TARGET="_blank">http://nationaldiagnostics.com..._id/6

hope this helps

chun


Libby

  • Guest
Re: What's the difference between 29:1 acrylamide:bis and 37.5:1 ... (cchan)
« Reply #2 on: January 15, 2007, 05:00:00 PM »
Hi Chun,

Thank you so much for the informative materails you presented.

Libby