Recent Posts

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91
DiPIA / Vaccine papers!
« Last post by GE Marie W on October 03, 2014, 05:38:29 AM »
Have you read these published papers about the use of Biacore systems in vaccine studies? I think they are really nice!

•   Method for the simultaneous assay of the different poliovirus types using  surface plasmon resonance technology. Vaccine 31 (2013) 1034–1039.
•   In-depth process understanding of RECOMBIVAX HB® maturation and potential epitope improvements with redox treatment: Multifaceted biochemical and immunochemical characterization. Vaccine 29 (2011) 7936–7941.
•   Structural basis for lack of toxicity of the diphtheria toxin mutant CRM197. Proc Natl Acad Sci U S A 2012 Apr 3;109(14):5229-34.

Happy reading!
Marie

92
DiPIA / Can't wait for the next DIPIA? Attend a Users Group Meeting!
« Last post by BrianGE on September 25, 2014, 08:33:17 AM »
We have several amazing meetings planned over the next few weeks.  Please visit www.biacore.com for more details.

September 26 in Cambridge, MA
October 16 in South San Francisco, CA
October 22 in Bethesda, MD

Keep checking in as we add more dates in more locations.

Brian
93
DiPIA / Re: Baseline drift
« Last post by GE anette on September 24, 2014, 02:18:05 PM »
Hi Curious,
We have no specific guidelines for basedrift and since you don't describe if the drift is going upwards or downwards and how much  baseline drift you obtain, this make it tricky to give a specific advice. As a general guidence I would say if you include positive controls during your assay or replicates of some samples, if these are coming back to the same response level you can feel quite confident that the activity of your immobilized molecule is keeping the activity and that the baseline drift can be ignored. If the controls or the replicates don't get the same response level the regeneration needs to be optimized.
Hope this can help
GE anette
94
DiPIA / Baseline drift
« Last post by Curious on September 23, 2014, 12:31:40 PM »
Hi,
My assay has a baseline drift probably due to not fully optimized regeneration. I wonder if there is any guideline how much drift is tolerated in assays?
Best regards
curious
95
DiPIA / Re: Biacore T200 for fragment screening
« Last post by GE Matt on September 22, 2014, 09:47:25 AM »
Hi Horatio:

In addition to the earlier comments, there was also a recent webinar that discusses various aspects of using SPR in fragment based drug discovery.  The webinar recording can be found here: https://www.youtube.com/watch?v=vl0Ec3MEbPU&list=UU8BPR_3mMsymuaJxo0HwvNg.
 
96
DiPIA / Re: Can you improve your analysis to obtain the information you need?
« Last post by GE Matt on September 22, 2014, 08:54:55 AM »
Looks like the link to this event is broken.  The video recording can be found here: https://www.youtube.com/watch?v=E-JeRMx1KZw&list=UU8BPR_3mMsymuaJxo0HwvNg.

97
DiPIA / Re: Use buffer with extra NaCl for Immunogenicity assays
« Last post by GE Anna M on September 22, 2014, 01:51:26 AM »
Another way of decreasing non-specific binding from serum that we often have found very efficient is to work with lower immobilization levels. If you want more information check out our publication on buffer optimization for plasma assays on Biacore.

Increased sensitivity of SPR assays in plasma through efficient parallel assay optimization,
Journal of Pharmaceutical and Biomedical Analysis, Volumes 78–79, 5 May 2013, Pages 224-232
Anna Moberg, Anna Lager, Markku D. Hämäläinen, Tanja Jarhede
98
DiPIA / Use buffer with extra NaCl for Immunogenicity assays
« Last post by GE Marie W on September 21, 2014, 10:09:47 PM »
Are you working with Immunogenicity assays?
Then we recommend you to use HBS-EP+ (that is with 0.05% detergent P20) and with extra added NaCl (500 mM NaCl, pH 7.4) as running buffer and optional as dilution buffer.
Kind regards
GE Marie
99
DiPIA / Is it possible to analyze binding to whole cells using Biacore instrument?
« Last post by GE Ewa on September 18, 2014, 08:19:05 AM »
Hi,

Recently, we successfully studied antibody interactions with cell surface proteins when whole Jurkat cells were attached to sensor surface of Biacore™ T200 instrument. Our results are briefly presented in a poster “Antibody analyses with Biacore™ system - convenient , sensitive, versatile”.
We would be happy to know whether you have any experiences on this application.  Share with us your thoughts or concerns, please.

Regards
Ewa
100
DiPIA / Connect, learn and share on DiPIA community
« Last post by GE Christina on September 15, 2014, 08:44:38 AM »
DiPIA Community allows you to share, learn and broaden your network in label-free interaction analysis.
Ask your question or share best practice here on the DiPIA forum
Follow DiPIA blog post and contribute with your comments and expertise
Get exclusive access to DiPIA conference materials and learn how to get the most out of your Biacore system in the Optimal interactions Club. Join DiPIA community today! www.gelifesciences.com/DiPIAdigital

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